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500 ml will remove 3 ppm of phosphate and silicate from roughly 16.000 liters (4226 gallons) of fresh water or 8.000 liters (2113 gallons) of marine water.
Overdosing can cause a negative effect in your tank due to rapid removal of phosphate and chemistry changes. It is recommended to use a small amount and gradually increase the amount of PO4x4 used, while monitoring the phosphate levels in your aquarium.
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